Peptide Reconstitution Calculator
Calculate bacteriostatic water volume, peptide concentration, and dose volume on a U-100 insulin syringe. Inputs and results update in real time.
Inputs
Edit either this OR the concentration below — they're linked.
Auto-calculated from water volume; type to override.
Total water
2
mL
Concentration
250
mcg / 0.1 mL
Doses per vial
20
injections
Volume per injection
0.1
mL · 10 units
U-100 Insulin Syringe
10 units · 0.1 mLEach tick = 1 unit (0.01 mL). Major labeled marks every 10 units (0.1 mL).
Quick Reference
Concentration in mcg per 0.1 mL by vial size and water volume. Your current selection is highlighted.
| Vial \ Water | 1 mL | 2 mL | 3 mL | 5 mL |
|---|---|---|---|---|
| 2 mg | 200 | 100 | 66.7 | 40 |
| 5 mg | 500 | 250 | 166.7 | 100 |
| 10 mg | 1000 | 500 | 333.3 | 200 |
How to Reconstitute Peptides
- 1Clean the vial stopper with an alcohol swab.
- 2Draw the calculated amount of bacteriostatic water into a syringe.
- 3Inject water slowly into the vial, aiming the stream against the glass wall — never directly onto the peptide powder.
- 4Gently swirl the vial — do not shake.
- 5Allow to dissolve completely (may take 1–5 minutes).
- 6Store reconstituted peptide refrigerated at 2–8°C.
- 7Note the date of reconstitution — most reconstituted peptides are stable for 4–6 weeks refrigerated.
Storage Guidelines
- Unreconstituted: room temperature or refrigerated, away from light.
- Reconstituted: refrigerated at 2–8°C, use within 4–6 weeks.
- Never freeze reconstituted peptides.