Peptide Reconstitution Calculator

Calculate bacteriostatic water volume, peptide concentration, and dose volume on a U-100 insulin syringe. Inputs and results update in real time — installable as an app for offline bench use.

Inputs

Load a peptide (autofills vial, water & a reference amount)

Preset vial sizes and volumes reflect how these compounds are commonly supplied and dissolved for laboratory research. Amounts are calculation reference points only — not dosing guidance or medical advice. Adjust any field freely.

Peptide amount in vial (mg)

Desired dose per injection (mcg)

Bacteriostatic water to add (mL)

Edit either this OR the concentration below — they're linked.

Desired concentration (mcg per 0.1 mL)

Auto-calculated from water volume; type to override.

Total water

Concentration

250

mcg / 0.1 mL

Doses per vial

injections

Volume per injection

0.1

mL · 10 units

U-100 Insulin Syringe

10 units · 0.1 mL

Each tick = 1 unit (0.01 mL). Major labeled marks every 10 units (0.1 mL).

Quick Reference

Concentration in mcg per 0.1 mL by vial size and water volume. Your current selection is highlighted.

Vial \ Water	1 mL	2 mL	3 mL	5 mL
2 mg	200	100	66.7	40
5 mg	500	250	166.7	100
10 mg	1000	500	333.3	200

How to Reconstitute Peptides

1Clean the vial stopper with an alcohol swab.
2Draw the calculated amount of bacteriostatic water into a syringe.
3Inject water slowly into the vial, aiming the stream against the glass wall — never directly onto the peptide powder.
4Gently swirl the vial — do not shake.
5Allow to dissolve completely (may take 1–5 minutes).
6Store reconstituted peptide refrigerated at 2–8°C.
7Note the date of reconstitution — most reconstituted peptides are stable for 4–6 weeks refrigerated.

Storage Guidelines

Unreconstituted: room temperature or refrigerated, away from light.
Reconstituted: refrigerated at 2–8°C, use within 4–6 weeks.
Never freeze reconstituted peptides.