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Catalog/HGH Fragment 176-191

HGH Fragment 176-191

Also known as HGH Frag 176-191 · hGH 176-191 · Somatropin fragment 176-191

The lipolytic C-terminal fragment of human growth hormone — the parent peptide that AOD-9604 was engineered from.

Research refreshed

Overview

HGH Fragment 176-191 is a 16-amino-acid piece of human growth hormone — residues 176 to 191, the C-terminal tail that carries GH’s fat-metabolizing activity. Isolated from the rest of the hormone, it is studied for lipolysis without the growth-promoting or blood-sugar effects of full GH, and it is the direct structural parent of the engineered analog AOD-9604.

Background

The idea behind HGH Fragment 176-191 is that growth hormone’s many effects are not all located in the same place on the molecule. Structure-activity work mapped GH’s fat-burning (lipolytic) activity to its C-terminal region, and the fragment spanning residues 176–191 was found to reproduce that effect on its own — while leaving behind the parts of GH responsible for growth signaling and the unwanted rise in blood glucose.

That makes the fragment a kind of natural experiment: a way to ask whether GH’s lipolysis can be separated from everything else it does. It is closed by a single disulfide bond between its two cysteines, and it is the peptide from which AOD-9604 was derived — AOD-9604 simply swaps the fragment’s N-terminal residue for a tyrosine to improve stability. It is a research compound and is not FDA-approved.

Mechanism

Proposed β3-adrenergic-mediated lipolysis; does not bind the GH receptor or raise IGF-1.

Key research findings

  • Lipolytic domain — reproduces the fat-metabolizing activity mapped to GH’s C-terminal region, isolated from the rest of the hormone.
  • No GH-receptor binding — studied for fat metabolism without the growth or glucose effects of full growth hormone or its IGF-1 rise.
  • Parent of AOD-9604 — the direct structural precursor; AOD-9604 adds an N-terminal tyrosine to the same fragment for stability.
  • Disulfide loop — a single Cys–Cys bond closes the peptide, its defining structural feature.
  • Preclinical — not FDA-approved as a therapeutic.

How HGH Fragment 176-191 is made

Behind every vial of HGH Fragment 176-191 is the same exacting pipeline every research peptide runs — but the chemistry plays out differently for this molecule. Here is how HGH Fragment 176-191, specifically, is brought into being.

  1. On paper first

    On paper, HGH Fragment 176-191 is C78H123N23O22S2 — about 1,799.1 daltons of precisely arranged atoms. Before a single bond is made, the target sequence, salt form, and purity threshold are written down as the contract the finished material must meet.

  2. Built residue by residue

    Assembling HGH Fragment 176-191 means roughly 16 coupling cycles on the synthesizer — one protected residue added at a time, which is also 16 chances for an incomplete coupling to seed a deletion impurity. It also carries a disulfide bridge, an extra step beyond a plain chain that adds both capability and cost.

  3. Purity is won here

    The crude mixture — HGH Fragment 176-191 plus its deletions and side products — is then separated on preparative HPLC, and where the cut is taken decides the difference between a genuinely pure peptide and a barely-passable one. HGH Fragment 176-191 carries 2 cysteines, whose thiols are oxidation-sensitive and can form disulfide links — reactive chemistry that purification has to control rather than ignore.

  4. Proven, then protected

    A real batch of HGH Fragment 176-191 proves itself: identity confirmed by mass spectrometry against its ~1,799.1 Da, purity read directly off an analytical HPLC trace, water and counterion content measured. That batch-specific certificate of analysis is the only honest way to know what is actually in a vial of HGH Fragment 176-191 — and a short, cold, accountable chain of custody is how that purity survives the trip to your bench.

Walk the full synthesis pipeline

Handling, storage & why purity is hard

The C-terminal hGH fragment (residues 176–191), made by solid-phase synthesis as a 16-residue peptide with a single disulfide loop between its two cysteines. The short chain assembles readily; forming and verifying that one disulfide correctly is the defining quality attribute — the same challenge as its analog AOD-9604.

Moderate synthesisDisulfide bridge

Don't judge a vial by its cake. A fluffy, good-looking lyophilized powder reflects bulking agents and freeze-drying parameters — not purity. Insist on a batch-specific certificate of analysis.

How peptides are made — the full pipeline

Research areas

  • Lipolysis
  • Obesity (preclinical)
  • Fat metabolism

Research-area guides

Frequently asked questions

What is HGH Fragment 176-191?+

It is a 16-amino-acid piece of human growth hormone (residues 176–191) that carries GH’s fat-metabolizing activity, studied for lipolysis without GH’s growth or blood-sugar effects.

How does it relate to AOD-9604?+

HGH Fragment 176-191 is the parent peptide; AOD-9604 is the engineered version, differing by an added N-terminal tyrosine that improves stability.

Does it raise IGF-1 like growth hormone?+

No — the fragment is studied specifically because it reproduces GH’s lipolytic effect without binding the GH receptor or driving IGF-1.

Is it approved?+

No — it is a research compound, not FDA-approved. This page is a research and educational reference.

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